Bertil Hille: from GPCRs to ion channels

Bertil Hille: from GPCRs to ion channels

Bertil Hille: from GPCRs to ion channels

  1. Full house at #HilleTalk Bertil Hille on GPCRs, ion channels and phospholipids. pic.twitter.com/60gkyVCgx3Cgx3
  2. More than 40% of prescription drugs act on GPCR pathways #HilleTalk
  3. I realize that my experiments are only in one cell and then I tell you about the whole brain, that is the way I see my results #HilleTalk
  4. Neonatal seizures, deafness, long QT associated to potassium channel #HilleTalk
  5. History of GPCR study. Cloning was what made thinks seem real. Sequencing put channel families together #HilleTalk
  6. Broad phenomena, many Gq coupled receptors affecting ion channels #HilleTalk
  7. How do Gq-mediated signals modulate ion channels? Focus on lipids! #HilleTalk
  8. Manipulating lipids to affect ion channel currents: VSP 5-phosphatase has same effect on KCNQ currents as GPCR #HilleTalk
  9. Using fluorescent markers to track phospholipid PIP2 on cell membrane. Oxo-M (GPCR) depletes PIP2 #HilleTalk
  10. What about neurons without receptor over-expression? Oxo-M have real depletion of PIP2, enough to explain m-current modulation #HilleTalk
  11. All Kv channels tested, only one sensitive to PIP2, in contrast all KCNQ are sensitive #HilleTalk
  12. What about Cav channels? Beta subunits tune PIP2 sensitivity. b2a is membrane anchored, may provide channel anchoring #HilleTalk
  13. Many other things sensitive to PIP2: Cytoskeleton, vesicle trafficking, not only ion channels #HilleTalk
  14. Concept: phosphoinositides are localized to specific membranes of the cell. Specific enzymes for organelles change composition. #HilleTalk
  15. Defect of phosphoinositides enzymes associated with lethal contractual syndrome, cataracts, glaucoma, liver fibrosis more. #HilleTalk
  16. In conclusion GPCRs change your state of mind and membrane phosphoinositides regulate proteins! #HilleTalk
  17. Thanks for following #HilleTalk, thanks to Bertil Hille for a great talk at @UW